FIG. 4.

Immunoprecipitations of the mutant S1 protein expressed in HeLa cells. Cells were infected with recombinant vaccinia virus vTF7-3 and transfected with the S1 gene. Cells were radiolabeled with [³⁵S]methionine and subjected to immunoprecipitation with selecting MAb HB10-4 (lanes 1, 2, and 3) or nonselecting MAb BB7-14 (lane 4). The immune complexes were resolved by SDS-polyacrylamide gel electrophoresis on 7.5% polyacrylamide gels, and the gel was autoradiographed. Lanes: 1, vTF7-3-infected but DNA-untransfected cell lysate; 2, wild-type BCV S1 gene, transfected; 3, A528V mutant S1 gene, transfected; 4, mutant S1 gene, transfected (A528V) and immunoprecipitated with MAb BB7-14.