Figure 8.

Effect of NB on p-gp expression and activity of PC3-DR cells. (A) Effect of NB on calcein accumulation (measures as green fluorescence) in PC3-DR cells. The effect was measures following 30 min incubation with the drug. P-gp inhibitor tariquidar (TQD, 50 nM) was used as a positive control. Number of replicates n = 3. (B) Western blotting analysis of protein expression in PC3-DR cells after 48 h of treatment with the indicated concentrations of NB. (C) Effect of NB on the viability of PC3-DR cells. Cells were treated with NB for 48 h with or without 30 min pretreatment with tariquidar (TQD, 50 nM) for 30 min. Viability was measured using an MTT assay. Number of replicates n = 3. Statistical significance: * p < 0.05 in ANOVA (A) or Student’s t-test (C).