Figure 9.

Combinational treatment on PC3-DR cells with NB and docetaxel. The cells were co-treated with indicated concentrations of NB, docetaxel, or their combination for 24 h. MTT assay was used to examine cellular viability. Number of replicates n = 3. (A) The heatmap represents cytotoxicity (viability inhibition) of the individual drugs or their combinations. (B,C) To calculate and visualize in 2D (B) or 3D (C) the effect of the drug combination (synergism/additive effect/antagonism), the SynergyFinder v. 2.0 software (Network Pharmacology for Precision Medicine in the Research Program of System Oncology, Faculty of Medicine at University of Helsinki, Helsinki, Finland) and a ZIP reference model were used. Green regions indicate antagonism, white regions refer to additive effect, and red regions indicate synergism.