Table 1.

Primers and their sequences used in this study.

Sequence Name	Gene Function	Sequence (5′-3′)	Ta (°C)	References
Act1	Housekeeping gene (Internal control)	F: GCTGGTAGAGACTTGACCAACCA R: GACAATTTCTCTTTCAGCACTAGTAGTGA	54	[27]
Eap1	Cell–cell adhesion, mediates adhesion to biotic and abiotic surfaces	F: CTGCTCACTCAACTTCAATTGTCG R: GAACACATCCACCTTCGGGA	54	[27]
Als3	Mediates attachment to host cells and matrix proteins	F: GGTTATCGTCCATTTGTTGA R: TTCTGTATCCAGTCCATCTT	54	[28]
Hwp1	Cell adherence	F: ACAGGTAGACGGTCAAGG R: GGGTAATCATCACATGGTTC	50	[28]
Bcr1	Transcription factor controls the als1, als3, hwp1, and hyr1 expressions	F: AATGCCTGCAGGTTATTTGG R: TTTTAGGTGGTGGTGGCAAT	50	[29]
Mkc1	Induced invasive filamentation and cell growth under stressed condition	F: AATGGGTCCAAAAAGGTTCC R: TTATGGCCCCTGAAGAACTG	50	This study