Table 1.

Overview of genes targeted in the single target CRISPR/Cas experiments. “Name” denotes the gene names used in this study and are underlined if they relate to the homologous gene in Saccharomyces cerevisiae.

Locus Tag	Name	Potential Function/Role	References
ACIB2EUKG769938	BGS5	Heavy chain dynein	[52]
ACIB2EUKG768596	BGS7	Pleckstrin-like, nuclear transport	[52]
ACIB2EUKG769034	BGS12	Cytoplasmic dynein, intermediate chain	[52]
ACIB2EUKG770622	BGS13 (PKC1)	Pc1 kinase, a protein serine/threonine kinase, which controls a highly conserved signaling pathway managing cell wall integrity	[51,52,53,54]
ACIB2EUKG771351	MSG5	Dual-specificity protein phosphatase (i.e., Ser/Thr- and Tyr-specific), which plays a role in the regulation of at least two mitogen-activated protein kinase (MAPK)-mediated pathways	[55]
ACIB2EUKG771893	YPK1	Ser/Thr-protein kinase and is a relevant part of sphingolipid-mediated and cell integrity signaling pathways	[55,56]
ACIB2EUKG770030	FKS1	1,3-beta-D-glucan synthase, therefore being responsible for the synthesis of the polysaccharide, which is the main structural component of the cell wall	[57]
ACIB2EUKG771759	ROM2	One of three guanine nucleotide exchange factors probably specific to Rho1p and Rho2p	[55]
ACIB2EUKG772498	NCE102	Integral membrane protein, being involved in an alternative pathway for protein export	[58,59]