Figure 3.

In vivo and in vitro decarboxylation of tryptophan and tyrosine catalyzed by DisA. (A) In vivo decarboxylation of tryptophan to tryptamine. Extracted ion chromatograms (EICs) of tryptophan (C₁₁H₁₃N₂O₂ 205.0977 [M + H]⁺, in red) and tryptamine (C₁₀H₁₃N₂ 161.1079 [M + H]⁺, in blue). (B) In vivo decarboxylation of tyrosine to tyramine. Extracted ion chromatograms (EIC) of tyrosine (C₉H₁₂NO₃ 182.0817 [M + H]⁺, in red) and tyramine (C₈H₁₂NO 138.0919 [M + H]⁺, in blue). In A and B, the base peak chromatogram of the extracts is given in grey. (C) Comparative HPLC analysis of the in vitro decarboxylation of tryptophan. (D) Comparative HPLC analysis of the in vitro decarboxylation of tyrosine. In C and D, the UV absorbance at 254 nm is shown.