Table 2.

Risk of Bias Assessment of Included Studies.

Study	Item 1	Item 2	Item 3	Item 4	Item 5	Item 6	Item 7	Item 8	Item 9	Item 10	Item 11	Item 12	Item 13	Item 14	Item 15	Overall
Asadi-Golshan, 2018	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	Low
Borhani-Haghighi, 2020	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	NR	✓	✓	NR	High
Cantinieaux, 2013	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	NR	Low
Chen, 2019	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	NR	Low
Chudickova, 2019	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	NR	Low
Cizkova, 2018	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	NR	✓	✓	NR	High
Gu, 2017	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	NR	Low
Kaneliyo, 2018	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	NR	✓	✓	✓	NR	Some concern
Khoshsirat, 2018	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	NR	Low
Tsai, 2019	✓	✓	✓	✓	NR	✓	✓	✓	✓	✓	✓	✓	✓	✓	NR	Some concern
Yeng, 2016	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	NR	Low

1. Species; 2. Using appropriate tests; 3. Severity of injury; 4. Level of injury; 5. Age/weight; 6. Number of animals per group; 7. Designation of strain; 8. Definition of control; 9. Description of statistical analysis; 10. Regulation and ethics; 11. Bladder expression; 12. Blindness of assessor; 13. Genetic background; 14. Method of allocation to treatments; 15. Description of the reasons to exclude animals from the experiment during the study (attrition).

✓: Low risk of bias; NR: Not reported