Table 2.
Table indexing retention times, pigment groups, absorbance maxima of each peak, and %III/II ratios that were observed in the chromatogram of spinach leaves.
| Peak nb | RT | Compounds | Absorption maxima | III/II | Spectrum | ||
|---|---|---|---|---|---|---|---|
| min | λ (nm) | % | quality | ||||
| 1 | 5.91 | chl dev | 468 | 600 | 654 | NA | + |
| 2 | 7.86 | chl dev | 468 | 652 | NA | - | |
| 3 | 8.95 | unknown | - | NA | – | ||
| 4 | 9.70 | chl dev | 434 | 620 | 668 | NA | ++ |
| 5 | 10.40 | unknown | - | NA | – | ||
| 6 | 11.15 | unknown | - | NA | – | ||
| 7 | 12.18 | car dev | 440 | NA | - | ||
| 8 | 12.45 | chl dev | 434 | 668 | NA | - | |
| 9 | 12.87 | car dev | 440 | 472 | NA | + | |
| 10 | 13.72 | car dev | 438 | 472 | NA | - | |
| 11 | 14.39 | car dev | 484 | NA | - | ||
| 12 | 14.87 | car dev | 434 | NA | ++ | ||
| 13 | 15.02 | car dev | 432 | 458 | 28.7 | + | |
| 14 | 15.49 | car dev | 440 | 470 | 94.3 | ++ | |
| 15 | 15.73 | chl dev | 420 | 666 | NA | - | |
| 16 | 16.37 | 9-cis-violaxanthin (VAZ) | 436 | 464 | 87.4 | ++ | |
| 17 | 16.60 | car dev | 444 | 472 | 53.8 | - | |
| 18 | 16.95 | car dev | 434 | 466 | 92.4 | - | |
| 19 | 17.06 | chl dev | - | NA | – | ||
| 20 | 17.46 | All-trans-neoxanthin (neo) | 440 | 470 | 91.8 | ++ | |
| 21 | 17.76 | unknown | - | NA | – | ||
| 22 | 18.16 | car dev | 420 | 448 | 91.9 | + | |
| 23 | 18.40 | car dev | 420 | 450 | NA | + | |
| 24 | 18.70 | unknown | - | NA | – | ||
| 25 | 19.10 | unknown | - | NA | – | ||
| 26 | 19.26 | unknown | - | NA | – | ||
| 27 | 19.62 | car dev | 442 | 468 | 63.8 | + | |
| 28 | 20.05 | car dev | 436 | 462 | 61.9 | + | |
| 29 | 20.25 | car dev | 436 | 462 | NA | - | |
| 30 | 21.18 | car dev | 446 | NA | - | ||
| 31 | 21.44 | lutein (lut) | 446 | 474 | 63.0 | ++ | |
| 32 | 22.22 | car dev | - | NA | – | ||
| 33 | 22.87 | chl dev | 420 | 666 | NA | – | |
| 34 | 23.13 | antheraxanthin (VAZ) | 446 | 474 | 57.7 | + | |
| 35 | 23.25 | all-trans-zeazanthin (VAZ) | 450 | 478 | 30.9 | + | |
| 36 | 23.46 | chl dev | 454 | 580 | 634 | NA | ++ |
| 37 | 23.67 | unknown | 454 | NA | – | ||
| 38 | 23.85 | chl dev | 454 | 632 | NA | - | |
| 39 | 23.90 | unknown | 454 | NA | - | ||
| 40 | 24.16 | chl dev | 454 | 644 | NA | - | |
| 41 | 24.45 | chl dev | 434 | 660 | NA | - | |
| 42 | 24.65 | chl dev | 436 | 660 | NA | - | |
| 43 | 24.82 | chl dev | 468 | 600 | 652 | NA | ++ |
| 44 | 25.22 | chl dev | 470 | 654 | NA | - | |
| 45 | 25.36 | chl dev | 434 | 658 | NA | - | |
| 46 | 25.59 | chl dev | 420 | 664 | NA | - | |
| 47 | 26.49 | chlorophyll b (chlb) | 468 | 602 | 652 | NA | ++ |
| 48 | 26.83 | chl dev | 420 | 656 | NA | - | |
| 49 | 27.15 | chl dev | 468 | 602 | 652 | NA | ++ |
| 50 | 27.51 | chl dev | 434 | 668 | NA | – | |
| 51 | 27.87 | chl dev | 428 | 666 | NA | – | |
| 52 | 28.05 | chl dev | 664 | NA | – | ||
| 53 | 28.45 | chl dev | 432 | 620 | 666 | NA | ++ |
| 54 | 29.17 | chl dev | 430 | 618 | 664 | NA | + |
| 55 | 29.47 | chl dev | 668 | NA | – | ||
| 56 | 30.31 | unknown | 474 | NA | - | ||
| 57 | 30.73 | chl dev | 668 | NA | – | ||
| 58 | 31.24 | chlorophyll a (chla) | 432 | 618 | 666 | NA | ++ |
| 59 | 33.07 | chl dev | 432 | 618 | 666 | NA | ++ |
| 60 | 43.94 | car dev | - | NA | – | ||
| 61 | 52.21 | α-carotene (α-car) | 446 | 472 | 61.3 | + | |
| 62 | 53.32 | β-carotene (β-car) | 456 | 476 | 13.6 | + | |