Fig. 1. RORγt⁺ APCs promote pT_reg differentiation and intestinal tolerance during early life.

a–d, Flow cytometry of RORγt and FOXP3-expressing CD4⁺ T cell subsets (a,c) and summary graphs (b,d) for frequencies of pT_reg (RORγt⁺FOXP3⁺) cells in mLN (a,b) and large intestine lamina propria (LI LP) (c,d) of 3-week-old MHCII^ΔRORγt (n = 7) and control (H2-Ab1^fl/fl) (n = 8) mice. e, Eight-week-old MHCII^ΔRORγt (n = 4) and control (n = 3) mice were analysed for frequencies of pT_reg (RORγt⁺FOXP3⁺) cells, RORγt^– T_reg cells and T_H17 (FOXP3⁻RORγt⁺) cells among CD4⁺ T cells in indicated tissues. f, Representative haematoxylin and eosin (H&E)-stained sections of colon from MHCII^ΔRORγt and control mice at 12 weeks of age. Scale bars, 200 μm. g, Histological colitis score in 12-week-old MHCII^ΔRORγt (n = 5) and control (n = 3) mice. Data are mean ± s.e.m. Each symbol represents an individual mouse. Data in a are pooled from two independent experiments. Data in e are representative of three independent experiments. Two-tailed unpaired t-test. *P < 0.05, **P < 0.01, ***P < 0.001 and ***P < 0.0001.

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