Fig. 4. Antigen presentation by ILC3s is not required for intestinal pT_reg differentiation.

a, Dot plot showing expression of genes related to antigen presentation, T cell priming and cell migration across Thetis cell and MHCII⁺ ILC3 clusters (Fig. 2b). b, Representative flow cytometry of mLN ILC3s (CXCR6⁺RORγt⁺MHCII⁺) and Thetis cells (CXCR6^–RORγt⁺MHCII⁺) from P18 Rorc^{Venus-creERT2}Aire^GFP mice (n = 3), showing expression of indicated chemokine receptors, co-stimulatory and immune-regulatory molecules. d,e, Immune cell composition of 3-week-old MHCII^ΔRORα (n = 3) and Rora^creH2-Ab1^fl/wt (n = 3) mice. d, Number of MHCII⁺ ILC3s and Thetis cells in mLN. e, Frequency of total T_reg (FOXP3⁺) and RORγt⁺ pT_reg cells. f, Frequency of total T_reg (FOXP3⁺), RORγt⁺ pT_reg cells and T_H17 cells in mLN and large intestine lamina propria (LI) of 12-week-old MHCII^ΔRORα (n = 5) and Rora^creH2-Ab1^fl/wt (n = 5) mice. g, Representative histological analysis of H&E-stained sections of the colon of mice in f (left) and summary histological colitis score (right). Scale bars, 200 μm. Data in b–f are representative of two or three independent experiments. Data are mean ± s.e.m. Each symbol represents an individual mouse. Two-tailed unpaired t-test.

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