Extended Data Fig. 2. Temporal ablation of MHC Class II on RORγt⁺ APCs.

a, Targeting strategy for the Rorc locus. b, Flow cytometry of Venus expression in thymocytes (left) or mLN TCRβ⁺CD4⁺ T cells (middle) and Lin^–CD90⁺CD127⁺ innate lymphoid cells (ILC; right) isolated from adult mice. c, Flow cytometry of mLN, LI and intestinal epithelial (IE) CD45⁺ and CD45^– cells in P16 Rorc reporter RORγt fate-mapper (Rorc^{Venus-creERT2}Rorgt^creRosa26^lsl-tdTomato) mice. Representative of n = 3 mice. d-e, Frequency of pTreg cells amongst CD4⁺Foxp3⁺ cells in mLN and LI (d) or frequency of RORγt⁺ APCs (Lin–RORγt(Venus)⁺MHCII⁺) (e) in mLN of Rorc^{Venus-creERT2}H2-Ab1^fl/fl (n = 4) or Rorc^{Venus-creERT2}H2-Ab1^fl/wt (n = 3) mice maintained on tamoxifen diet from 8–13 weeks of age. Each symbol represents an individual mouse. Data in b–d representative of two independent experiments. Error bars: means ± s.e.m.; *P < 0.05; unpaired two-sided t-test.

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