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. 2022 Sep 7;610(7933):722–730. doi: 10.1038/s41586-022-05266-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a, Shown are graphs for consumption (AceK and IL) in two-bottle 48 h preference assay for control and cNST-silenced animals (n ≥ 8 mice), P = 0.151 (from Fig. 2a). b, Consumption in two-bottle 48 h preference assay for control and Vip-silenced mice (n ≥ 7 mice), P = 0.69 (from Fig. 4b). c, Consumption in two-bottle 48 h preference assay for control and Trpa1-silenced mice (n ≥ 6 mice), P = 0.44 (from Fig. 5c). Values are mean ± s.e.m. d–f, Animals with genetically silenced sugar/nutrient preference vagal neurons (VIP), or fat-only vagal neurons (Trpa1) still exhibit normal innate attraction to sweetener (d), sugar (e), and fat stimuli (f). Shown are graphs for 30 min two-bottle tests for control mice, and for mice with silenced VIP-expressing vagal neurons (VIP-Tx) and mice with silenced Trpa1-expressing vagal neurons (Trpa1-Tx). d, AceK versus water in VIP-Tx (n = 8) and Trpa1-Tx (n = 6) animals is not significantly different from controls. ANOVA with Tukey’s test: VIP-Tx, P = 0.36, Trpa1-Tx, P = 0.66. e, Glucose versus water in VIP-Tx (n = 8) and TrpA1-Tx (n = 6) is not significantly different from control animals. ANOVA with Tukey’s test: VIP-Tx, P = 0.45, Trpa1-Tx, P = 0.67. f, IL versus water in VIP-Tx (n = 8) and TrpA1-Tx (n = 6) is not significantly different from control animals. ANOVA with Tukey’s test: VIP-Tx, P = 0.87, Trpa1-Tx, P = 0.91. Values are mean ± s.e.m. Tastants: AceK (3 mM), Glucose (200 mM), IL (1.5%). g, The graph shows body weight measurements from Vip-Cre animals injected with AAV-Flex-TetTox in both nodose ganglia, from the time the animals were infected until the time behavioral preference tests were performed (days 24–26.); data is presented as percent change, with weight at time zero defined as 100%. Thin lines represent individual animals; dark lines represent the average body weight of TetTox (n = 7 mice, red) and control (n = 10 mice, black) animals. No significant differences were detected, two-way ANOVA, P = 0.37.