REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
S. aureus	(Novick et al., 1993)_	RN6390
S. aureus	(Horsburgh et al., 2002)	SH1000
S. aureus	(Fey et al., 2013)	USA300 JE2
S. aureus	(O’Neil et al., 2007)	BH1CC
S. aureus	(Holden, 2010)	TW20
S. aureus	(Castillo-Ramírez et al., 2012)	HU6
S. aureus	(Castillo-Ramírez et al., 2012)	HU24
S. aureus	(Castillo-Ramírez et al., 2012)	IU4
S. aureus	(Castillo-Ramírez et al., 2012)	IU9
S. aureus	(Castillo-Ramírez et al., 2012)	IU12
S. aureus	(Castillo-Ramírez et al., 2012)	DEU9
S. aureus	(Castillo-Ramírez et al., 2012)	DEU19
S. aureus	(Castillo-Ramírez et al., 2012)	DEU20
S. epidermidis	This laboratory	1455
E. coli	This laboratory	MG1655
P. aeruginosa	This Laboratory	PA01-N
S. pyogenes	(Scott et al., 1986)	JRS4
S. agalactiae	(Valenti-Weigand et al., 1996)	6313
C. difficile	Ed Kuijper Collection	8085054
C. difficile	Ed Kuijper Collection	8079089
B. subtilis	Bacillus Genetic Stock Centre	168
Chemicals, peptides, and recombinant proteins
Müller–Hinton broth	Oxoid	Cat# CM0405
L-cysteine	Oxoid	Cat# CM1135
Brain Heart Infusion broth	Oxoid	Cat# LP0021
Yeast Extract	Sigma-Aldrich	Cat# C7352
C. difficile supplement	Oxoid	Cat# SR0096
Luria Broth	Oxoid	Cat# CM0996B
Defibrinated Rabbit Blood	TCS biosciences	Cat# RB052
Phosphate Buffered Saline	Sigma-Aldrich	Cat# P4417
Triton X-100	Sigma-Aldrich	Cat# T8787
Resazurin	Sigma-Aldrich	Cat# R7017
RPMI-1640 (Biofilm Studies)	Lonza	Cat# 12-918F
RPMI-1640 (Cell Culture)	Sigma-Aldrich	Cat# R7509
Penicillin Streptomycin mix	Sigma-Aldrich	Cat# P0781
Glutamine	Sigma-Aldrich	Cat# G7513
Fetal Bovine Serum	Sigma-Aldrich	Cat# F9665
Tobramycin	Sigma-Aldrich	Cat# T04014
Propidium Iodide	Sigma-Aldrich	Cat# P4170
Nisin	Sigma-Aldrich	Cat# N5764
di-8-ANEPPS	Thermo-Fisher	Cat# D3167
Disc3(5)	Thermo-Fisher	Cat# D306
Valinomycin	Sigma-Aldrich	Cat# V0627
Critical commercial assays
LDH Cytotoxicity Assay Kit	Biochain	Cat# K6330400
Cell Titre-Glo kit	Promega	Cat# G7570
Experimental models: Cell lines
Jurkat T cells	Stratech	PC-006-SIG
Recombinant DNA
pMMR	(Popat et al., 2012)	Constitutive mCherry fluorescent reporter
pBK-miniTn7-egfp	(Popat et al., 2012)	Constitutive eGFP fluorescent reporter
Software and algorithms
ImageJ	(Schneider et al., 2012)	https://imagej.nih.gov/ij/
Zen	Zeiss, Germany	www.zeiss.com
Graphpad Prism 8.0	GraphPad Software, USA	https://www.graphpad.com