TABLE 1.
Comparison of calculated amounts of IFN-γ using the Q-IFN ELISA with two different methods of standard curve development
Absorbance | Actual concn of IFN-γ (IU/ml) | IFN-γ concn (IU/ml) from the standard curve by the indicated method
|
|
---|---|---|---|
Point-to-point | Best linear fit | ||
3.531 | 137.2 | 149.3 | 142.6 |
2.134 | 69.6 | 75.2 | 80.5 |
1.346 | 34.8 | 38.6 | 45.5 |
0.49 | 8.4 | 7.9 | 7.5 |
0.358 | 4.2 | 4.2 | 1.6 |
0.148 | 0 | −0.1 | −7.7 |