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. 2001 Mar;8(2):339–345. doi: 10.1128/CDLI.8.2.339-345.2001

TABLE 1.

Comparison of calculated amounts of IFN-γ using the Q-IFN ELISA with two different methods of standard curve development

Absorbance Actual concn of IFN-γ (IU/ml) IFN-γ concn (IU/ml) from the standard curve by the indicated method
Point-to-point Best linear fit
3.531 137.2 149.3 142.6
2.134 69.6 75.2 80.5
1.346 34.8 38.6 45.5
0.49 8.4 7.9 7.5
0.358 4.2 4.2 1.6
0.148 0 −0.1 −7.7