Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 26;12:17999. doi: 10.1038/s41598-022-22851-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Production of IgA plantibody specific for Stx1B in a plant expression system. (a) Schematic diagram of the transfer-DNA (T-DNA) region of the Stx1B-specific dimeric IgA expression vector (pRI201/dimeric IgA). P_LHCB, light-harvesting complex II (LHCB) promoter; T_1.1 and T_1.3, LHCB terminators; Hc, IgA heavy chain gene; Lc, IgA light chain gene; Jc, immunoglobulin joining chain gene; 35S, cauliflower mosaic virus 35S promoter; T_HSP, terminator of Arabidopsis heat shock protein 18.2 gene; nptII, neomycin phosphotransferase II gene; LB, left border of T-DNA region; RB, right border of T-DNA region. Immunoglobulin domain organizations of the heavy chain inserts for the hyIgA and IgA are shown in parallel. VH, variable region; Cγ, heavy chain constant regions from IgG1; Cα, those from IgA; Hinge (γ or α), hinge region from IgG1 or IgA; KDEL, the endoplasmic reticulum retention signal peptide. (b–g) Immunoblot analysis of leaf extracts of transgenic Arabidopsis thaliana. Leaf proteins (b,c,e,f, 10 ng/lane as IgA; d,g, 30 ng/lane as IgA) were separated by SDS-PAGE under reducing (b–d: 12% gel) and non-reducing (e–g: 4–15% gradient gel) conditions, and then blotted onto PVDF membranes. Each immunoglobulin chain was detected with anti-IgA heavy chain (b,e), anti-κ light chain (c,f), and anti-J chain (d,g) antibodies, respectively. Arrows indicate the intact immunoglobulin chains and arrowheads indicate the incomplete IgA heavy chains. Leaf extracts of Stx1B-specific IgA transgenic A. thaliana (IgA) or hyIgA transgenic A. thaliana (hyIgA) were analyzed.