Skip to main content
. 2022 Oct 26;13(10):901. doi: 10.1038/s41419-022-05333-3

Fig. 7. FAM134B functions downstream of GSDMD/ERS to regulate DIC.

Fig. 7

A Survival curve of GSDMD(flox/flox) and GSDMD-CKO mice after DOX and 4PBA treatments was created by Kaplan–Meier method. N ≥ 15 per group. B, C Representative echocardiograms from GSDMD(flox/flox) and GSDMD-CKO mice treated with DOX and 4PBA are shown. Heart rate (HR), ejection fraction (EF) and fractional shortening (FS) are measured by echocardiography. N = 7 per group. D, E Western blot detection of Cleaved-caspase 3 (Cleaved cas3), BAX, LC3, Bip, and FAM134B protein abundance from mice after DOX and 4PBA treatments. Quantification of protein abundance as illustrated in panel. N = 6 per group. F–H Western blot detection of Cleaved-caspase 3 (Cleaved cas3), BAX, LC3, Bip, and FAM134B protein abundance in cardiomyocytes after DOX treatments. Quantification of protein abundance as illustrated in panel. N = 6 per group. I Working model by which how GSDMD induces cardiotoxicity after DOX administration. Data are depicted as the mean ± SEM. Statistical significance was determined by one-way and two-way ANOVA with a post hoc Holm–Sidak test, ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.