Fig. 2.

ACR16 and PRE-084 increase the number of synapses in 12 DIV rat hippocampal primary cell cultures. Confocal fluorescence microscopy of immunolabeled hippocampal primary cell cultures following 48 h exposure to different concentrations of σ1R agonists, as indicated; VGLUT1 (red) and synapsin1 (green), scale bar = 10 μm. The discrete areas with double-immunoreactivity were identified as synapses and quantified. The adjacent graph displays the results of the count for each experimental condition and each individual experiment [mean (Min–Max); n = 5]. Results are expressed as the mean ± min to the max, where each dot represents a single experimental value. Statistical analysis was performed using 1-way ANOVA (F (4,20) = 10.13; ***p < 0.001) and Tukey’s multiple comparisons post-test. Significant differences compared with control controls are expressed as *(p < 0.05), **(p < 0.01), and ***(p < 0.001), respectively