FIG. 1.

WB of supernatant and dissolved pellet fractions of PEG-derived CAEV whole-virus antigens at different TCS days, following precipitation, dialysis, and concentration with PEG 8000. Lanes 1 to 3, PEG-derived antigen fractions from the infected dissolved pellet of TCS days 25 (lane 1), 23 (lane 2), and 19 (lane 3); lanes 4 to 5, PEG-derived antigen fractions from the infected supernatant of TCS days 23 (lane 4) and 19 (lane 5); lane 6, PEG-derived fraction from TCS day 19 of noninfected cells. A reference CAEV-positive goat serum recognizing the capsid antigen of the virus was used. A specific band of around 25 kDa was detected in lanes 1 to 5.