FIG. 3.

WB of supernatant and dissolved pellet fractions of PEG-derived CAEV whole-virus antigen batch 1 following precipitation, dialysis, and concentration with PEG 8000. Lanes 1 and 2, dissolved pellet (lane 1) and supernatant (lane 2) fractions of PEG-derived antigens from eight roller bottles at TCS day 23 of infected cells; lane 3, dissolved pellet fraction of noninfected cells. A reference polyclonal CAEV-positive goat serum was used. Specific bands were detected at approximately 10, 25, and 42 kDa. Also, two other bands were detected between 25 and 42 kDa.