Figure 2.

Blockade of FcγRIV increases neutrophil count in the skin of mice with experimental EBA. Using multiphoton microscopy, infiltrating dermal neutrophil numbers in experimental EBA treated with anti-FcγRIV Ab or isotype control were evaluated on days 1, 3 and 8. For every mouse and day, 7 image stacks (250 x 250 x 90 µm) were acquired and analyzed. (A) Representative images are the bird’s-eye view of a full stack projection on days 1, 3 and 8. (B) Individual values for counted stacks and the resulting mean ±SD are plotted for both experimental groups (n = 5/group). Overall, significantly more neutrophils were detected in FcγRIV Ab-treated mice compared to isotype control-treated mice on day 1 (p = 0.0001), day 3 (p=0.0095) and day 8 (p < 0.0001). ** <0.05, *** <0.001.