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Cloning of optimized vaccine sequences in the pET28a (+) expression system. (A) Cloning of the L1R vaccine sequence in the pET28a (+) vector. (B) Cloning of the B5R vaccine sequence in the pET28a (+) vector. (C) Cloning of the A33R vaccine sequence in the pET28a (+) vector. (D) Cloning of the proteome-wide vaccine sequence in the pET28a (+) vector.
