| PURPOSE | SEQUENCE (5′ to 3′) | |
|---|---|---|
| ssODN with 1xFLAG and 1xHA | CRISPR/Cas9 |
ATCTTAGGCGGTTCAGATCCCGGAAGGAAAAAGCCCAGCCTACTCCGAGG GACTACAAAGACGATGACGACAAGGGGTATCCCTATGACGTCCCGGACTATGCA TAGAAAGGTTACCGGGAATTGTCAGCAGCTCCAATGCCTGCCCTGAAGTC |
| sgRNA Template (T7 Forward) | CRISPR/Cas9 |
GAAATTAATACGACTCACTATAGGCAGCCTACTCCGAGGTAGAAGTTTTAGAGCTA GAAATAGC |
| sgRNA Template (T7 Reverse) | CRISPR/Cas9 |
CAAAATCTCGATCTTTATCGTTCAATTTTATTCCGATCAGGCAATAGTTGAACTTTTTC ACCGTGGCTCAGCCACGAAAA |
| Brwd1 Common Primer (Forward) | Genotype, RT-qPCR | TGCATAGTGACACCCTGAAAG |
| Brwd1 Common Primer (Reverse) | Genotype, RT-qPCR | 3′-GCTGTGTAGAGCTAACTGGAAG-5′ |
| Epitope-Specific Primer (Reverse) | RT-qPCR | 3′-TCCGGGACGTCATAGGGATA-5′ |
| Brwd1 repro5 Primer (Forward) | Genotype | ATGGCCACTGTAGGTTCAGC |
| Brwd1 repro5 Primer (Reverse) | Genotype | TTAAAGTCCACGACCCCTGA |
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