Figure 1.

Cancer cells consume BCAAs and secrete BCKAs. (A), BCKAs (KIV, KIC and KMV) in the DMEM complete medium or conditioned media (CM) from indicated cells were detected by GC-MS. (B), Representative mass spectra of KIV, KIC and KMV by GC-MS. (C), BCAAs (leucine, isoleucine and valine) in the DMEM complete medium or conditioned media from indicated cells were detected by GC-MS. (D), Diagram of workflow. Conditioned media from cancer cells and three BCKAs were added to BMDM or iBMDM, followed by multiomics analysis. Data in A and C show the mean ± SEM of n = 3 technical experiments.