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. 2022 Oct 26;12:17948. doi: 10.1038/s41598-022-22873-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Microbial community profiling and absolute quantification of pilot donation fluid. (a) Lactobacillus colony forming unit (CFU) quantification for each donation and analysis aliquot. Total donation volume is listed below donation numbers. (b) Microbial species in donation (“D”) and analysis (“A”) aliquots of pilot donor (Donor 0) samples using bacterial 16S rRNA amplicon sequencing. D1 and D2 refer to two different donation aliquots from the same donation collection.