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. 2022 Oct 26;12:17948. doi: 10.1038/s41598-022-22873-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Microbial community profiles of VMT donation samples. (a) Microbial community and metadata. Relative abundance of the bacterial community in the donation material was determined using 16S rRNA amplicon sequencing for each donation. Nugent score and white blood cell (WBC)/epithelial cell ratio are presented below each donation. (b) Absolute quantification of L. crispatus and L. iners in donation samples using species-specific qPCR. Detection limit of the assay is depicted by dashed lines. (c) Donation stability measured as Lactobacillus CFUs streaked and counted on MRS agar at long-term intervals post freezing.