Figure 5.

LBR localization, nuclear morphology in fibroblasts, and Pelger-Huët-like anomaly in granulocytes

(A) Localization analysis of LBR and actin in fibroblasts from i1, i22, and i23 and healthy control individuals (only control 1 is shown in the figure). Scale bar, 20 μm. The inset in control 1 line shows a higher magnification of the nucleus indicated by the dashed square. Actin staining shows that TMEM147 does not affect gross cell morphology.

(B) Nuclear segmentation is observed in fibroblast cell nuclei from affected individuals. Fibroblasts were stained with the May Grunwald-Giemsa (MGG) method.

(C) Quantification of the nuclear segmentation observed in fibroblast cell lines.

(D) Chromatin clumping, hyposegmentation, bilobed nuclei, carioschizes, and drumsticks (red arrows) observed in neutrophils of i1.