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E1 has no effect on IL-10 expression neither in in vitro stimulated THP-1 macrophages nor in primary WAT SVF. (A) Relative IL10 mRNA expression in E1, TS, E2 and TNFα-treated THP-1 macrophages. Two-way ANOVA with Sidak’s multiple comparison was used to test the significance. ** <0.01 (n=4). (B) Relative IL10 mRNA in E1-stimulated WAT SVF; (Mann-whitney test, n=4 subjects in triplicates).
