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. 2022 Oct 13;12:975408. doi: 10.3389/fonc.2022.975408

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Copyright © 2022 Deng, Yang, Tian, Liu, Sun and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OX40L mRNA elicited lymphocyte activation in the H22 tumor bearing mice. (A, C) T-cell activation by flow cytometry. CD4⁺ T cells and CD8⁺ T cells in the spleen of the H22 tumor-bearing BALB/c mice. (B, D) Quantitative analysis of the proportions in (A, C). Statistical significance was calculated via t test. **P ≤ 0.01, ***P ≤ 0.001. (E, F) Representative FACS profiles of CD69⁺ CD8⁺ and CD69⁺ CD4⁺ activated T cells. (G) Results of quantitative analysis of the proportions in (E, F). Statistical significance was calculated via t test. *P ≤0.05. (H) Immunohistochemistry of the mechanisms of action of OX40L mRNA in subcutaneous H22 tumors (scale bars: 100μm).