Table 1.
CAI | GC% | Negativecis-acting elements | Negative repeat elements | |
---|---|---|---|---|
Optimized OX40L sequence | 0.93 | 55.7 | 0 | 0 |
Original OX40L sequence from NM_003326 | 0.54 | 46.3 | 1 | 0 |
aCodon usage bias was adjusted to fit the highest expression profile of the target host. A CAI (Codon Adaptation Index) of 0.8 - 1.0 is regarded as good for high expression.
bRepeated area in the original sequences were removed to avoid stem-loop structures in mRNA and facilitate the synthesis process.
cUndesired motif including restriction enzyme site to be used in sub-cloning and negative cis-acting sites were modified.
dWhole sequence was fine-tune to increase the translation efficiency and prolong the half-life of mRNA.