Skip to main content
. 2022 Sep 26;109(10):1814–1827. doi: 10.1016/j.ajhg.2022.09.003

Figure 5.

Figure 5

Human RABEP2-coding variants impair cell migration in an in vitro scratch wound assay

(A) Representative bright-field images display four different groups: control infected with CMV-control after control siRNA treatment, loss of function (LoF) infected with CMV-control after si-RABEP2 treatment, rescued infected with CMV-RABEP2 after si-RABEP2 treatment, and p.Arg543His infected with CMV-RABEP2-p.Arg543His after si-RABEP2 treatment. A fully confluent (100%) HMEC monolayer for each treatment was scratched with the tip of a 200 μL pipet. At different time intervals (0, 4, 8, and 10 h), the degree of migration of HMECs was imaged.

(B) The line graph shows wound closure ratio. The wound closure rate was quantified as the percentage of area reduction at each time point. The total number of scratch experiments for control, LoF, rescued, p.Arg508Ser, p.Ser204Leu, p.Arg490Trp, and p.Arg543His were 15, 16, 12, 19, 15, 9, and 31, respectively. Data represent the mean ± SD and statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test (∗p < 0.05; ### (individual group) p < 0.001 versus control and rescued groups; NS, not significant).

(C) The scatterplots show the percentage of wound closure 10 h after scratch. Data represent the mean ± SD and statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test (∗∗∗p < 0.001).