FIG. 1.

(Top) Protein profiles of B. burgdorferi sensu lato isolates after polyacrylamide gel electrophoresis and Western blot on polyvinylidene difluoride membrane stained with Coomassie blue. Representative isolates of each Borrelia genospecies are ordered according to their RFLP pattern. Sizes are shown in kilodaltons. Lanes 1 to 3, B. burgdorferi sensu stricto (VS215, VS619, and NE49). Lanes 4 to 6, B. bissettii (CA128, DN127, and 25015). Lane 7, B. andersonii (21123). Lanes 8 to 11, B. garinii (VS102, VSDA, AS19s, and NT29). Lane 12, B. afzelii (VS461). Lane 13, B. valaisiana (VS116). Lane 14, B. japonica (FiAE2). Lanes 15 and 16, B. lusitaniae (PotiB3 and IR345). (Bottom) Reactivity with monoclonal antibodies. (+), weak reactivity.