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. 2022 Oct 13;13:992626. doi: 10.3389/fimmu.2022.992626

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Copyright © 2022 Gao, Lu, Yan, Wang, Xia, Wang, Li, Chang and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the relationship between AURKA, SHC1, and NAPSA genes and radiotherapy sensitivity, and autophagy by constructing resistant cell lines compared with primary cells. (A) Colony formation assay reveal constructed resistance cells with enhanced radioresistance. (B) The western blotting assay showed increased levels of resistant cell autophagy. (C, D) Quantitative Real-time PCR verified significant differences in radiotherapy autophagy-related genes (AURKA, SHC1) between radiotherapy-resistant and primary cells. *p < 0.05, **p < 0.01, and ***p < 0.001. "ns" indicates P value ≥ 0.05, not statistically significant.