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. Author manuscript; available in PMC: 2022 Oct 27.
Published in final edited form as: Matrix Biol. 2022 Feb 12;107:77–96. doi: 10.1016/j.matbio.2022.02.002

Figure 8. Loss of Pofut2 minimally impacted secretion of CCN2 and ADAMTS17 in developing tibia.

Figure 8.

(A-H’) Maximum projection images of E14.5 hind limb sections from control (Pofut2-Flox/+;Prrx1-Cre/+) (A-D’, K-N’) and Prrx1-Pofut2 mutant (Pofut2-Flox/Δ;Prrx1-Cre/+) (E-H’, O-R’) embryos immunostained with CCN2 (red) and BiP (green) and counterstained with DAPI (blue) (A-H’) or immunostained with ADAMTS17 and BiP and counterstained with DAPI (K-R’). (A and E) Lower magnification images showing all channels with white boxes indicating regions of higher magnification showing CCN2 (red) (B and F), BiP (green) (C and G), and DAPI (blue) (B-C). (D-D’ and H-H’) Merged channels with digitally enlarged panels (D’ and H’). (I-J) Quantification of CCN2 (I) and BiP (J) immunofluorescence signals in defined regions of proliferating zone (PZ), prehypertrophic zone (PHZ) and hypertrophic zone (HZ) cells (see Fig. S17). (K-R’) Lower magnification images showing ADAMTS17 (red), BiP (green), and DAPI (blue) with white boxes indicating regions of higher magnification showing ADAMTS17 (L,P), BiP (M,Q), and DAPI (M-N, Q-R). (N-N’ and R-R’) Merged channels with digitally enlarged panels (N’ and R’). (S-T) Quantification of ADAMTS17 (S) and BiP (T) immunofluorescence signals in defined regions PZ, PHZ, HZ and perichondrium (PC) cells (see Fig. S18). Analyses were performed using a single hind limb isolated from minimum of three embryos per genotype (n=3) and three sections per limb. Data from control (red dots) and Prrx1-Pofut2 mutants (black dots) were evaluated for statistical significance using unpaired, two-tailed t-test: *p≤0.05, **p≤0.01 and NS; not significant (E, F, K and L). Scale bars: 50 μm.