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. 2022 Oct 13;16:1020644. doi: 10.3389/fncel.2022.1020644

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Copyright © 2022 Gong, Zhang, Ren, Zhang, Zhao, Mao, Gan, Wang, Ma, Lin, Ye, Qian and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electroacupuncture (EA) at the head acupuncture points had a more significant effect on promoting BBB opening than the non-head points. (A) Schematic diagram of EA at different points. (B) The first row shows the degree of brain EB penetration of each group under naked eye observation. Distribution of brain EB in each group was observed using IVIS (Color Scale: Min = 2.25 e⁹, Max = 2.96 e¹¹). The third row shows the fluorescence intensity and distribution of EB in each group under the brain sagittal slice. (C) EB fluorescence intensity of cerebral cortex in each group under IVIS; **P < 0.01, GV20 + GV26 group vs. control group, ST36 group; P > 0.05, control group vs. ST36 group, n = 6 per group. (D) EB concentration in the cerebral cortex of each group; ***P < 0.001, GV20 + GV26 group vs. control group and ST36 group; P > 0.05, control group vs. ST36 group, n = 6/group.