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. 2022 Oct 13;9:1028867. doi: 10.3389/fvets.2022.1028867

Figure 5.

Figure 5

Analysis of miR-664b sponge HSD17B7 3'-UTR and lncRNA NONBTAT027373.1. (A): The networks of lncRNA-miRNA-HSD17B7 (B): The expression of DE lncRNAs in lncRNA-miRNA-HSD17B7 networks. (C): The expression of HSD17B7 mRNA. (D): The expression of DE miRNAs in lncRNA-miRNA-HSD17B7 networks. (E): Interaction between lncRNA NONBTAT027373.1 and miR-664b through Ago2 protein investigated using the RIP assay. lncRNA NONBTAT027373.1 and miR-664b expression quantified by RT-qPCR. (F): Schematic view of the miR-664b seed region, the motif of HSD17B7 3'-UTR and lncRNA NONBTAT027373.1. (G,H): Construction of WT and MUT plasmid vectors of HSD17B7 3'-UTR and lncRNA NONBTAT027373.1. (I): Dual-luciferase reporter gene analysis of the regulatory relationship between HSD17B7 3'-UTR and miR-664b. (J): Dual-luciferase reporter gene analysis of the regulatory relationship between lncRNA NONBTAT027373.1 and miR-664b. (K): Transfection efficiency of miR-664b after overexpression and knockdown of miR-664b. (L): miR-664b regulates the expression of HSD17B7 mRNA. (M): RT-qPCR analysis of the effect on silence of lncRNA NONBTAT027373.1 expression by siRNA. (N): Relative expression of HSD17B7 mRNA in groups. NS, Non-significant. Values represent means ± SEM for three individuals. *P < 0.05, **P < 0.01. Yellow nodes represent HSD17B7; red nodes represent miRNAs and blue nodes represent lncRNAs in lncRNA-miRNA-HSD17B7 networks. WT, Wild Type; MUT, Mutant; Mimics, Overexpression of miR-664b; Inhibitor, Knockdown of miR-664b; NC, Negative control.