TABLE 1.

Genetic zebrafish models of PD.

Study	Method(s)	DA neuron loss	Other pathologies	Motor deficits	Other phenotypes
Anichtchik et al., 2008	PINK1 MO knockdown	Yes	ROS accumulation	Yes – Impaired TEER	Morphological deformities. Increased mortality.
Bretaud et al., 2007	DJ-1 MO knockdown	No	Increased sensitivity to oxidative stress. Increased sensitivity to proteasome inhibition. Upregulation of apoptosis regulator genes	Not reported
Fett et al., 2010	Parkin MO knockdown	No	Increased susceptibility to proteotoxic stress	Not reported
Flinn et al., 2009	Parkin MO knockdown	Yes	Reduced mitochondrial activity. Increased susceptibility to MPP+	No
Flinn et al., 2014	TILLING-mediated PINK1 knockout	Yes	Mitochondrial dysfunction. Increased microglial numbers and activation.	Not reported
Lulla et al., 2016	γ1-synuclein overexpression	Not reported	Synuclein aggregation	Not reported	Morphological deformities. Increased mortality.
Milanese et al., 2012	β-, and γ1-synuclein MO knockdown	Yes		Yes – Reduced spontaneous swim activity
Prabhudesai et al., 2012	Human α-synuclein overexpression	gross neuronal apoptosis	Synuclein aggregation	Not reported	Developmental deformities. Embryonic lethality (100%).
Prabhudesai et al., 2016	LRRK2 MO knockdown	Yes	Synuclein aggregation	Not reported	Morphological deformities.
Priyadarshini et al., 2013	PINK1 MO knockdown, Microarray analysis	Not reported	Significant alteration of 177 genes. Increased ROS levels	Not reported	Reduced heart rate. Increased erythropoiesis.
Ren et al., 2011	ΔWD40-LRRK2 MO knockdown	No		No
Sallinen et al., 2010	PINK1 MO knockdown	Yes	Increased vulnerability to MPTP exposure	Yes (post-MPTP treatment) – Reduced swim activity
Sheng et al., 2010	LRRK2 MO knockdown	Yes		Not reported	Morphological deformities. Embryonic lethality (∼90%).
Sheng et al., 2010	ΔWD40-LRRK2 MO knockdown	Yes	Reduction and disorganization of axon tracts	Yes – Reduced swim activity
Suzzi et al., 2021	LRRK2 CRISPR/Cas9-mediated knockout	No – But general increase in apoptosis	Reduced mitosis in larval brains. Impaired neuronal regeneration in adult brains.	Yes – Reduced spontaneous swim activity
Xi et al., 2010	PINK1 MO knockdown	No – But disrupted patterning of the diencephalc DA neurons		Yes – Deficient TEER; reduced swim distance and speed; increased turning angle; preference to periphery of observation dish; difficulties in balancing	Increased mortality.
Zhao et al., 2012	FBXO7 MO knockdown	Yes		Yes – Reduced swim velocity	Morphological deformities. Increased mortality.