TABLE 2.

Chemical zebrafish models of PD.

Study	Method(s)	DA neuron loss	Other pathologies	Motor deficits	Other phenotypes
Babu et al., 2016	MPTP	Not reported		Yes – Reduced swim movement and distance; reduced number of crosses; increased number of freezing bouts and duration
Benvenutti et al., 2018	6-OHDA	Not reported		Yes – Reduced swim distance, speed, and maximum acceleration; increased absolute turn angle; increased immobility time	Reduced head and total length.
Bortolotto et al., 2014	Paraquat	Not reported		Yes – Reduced swim distance and velocity; impaired motor coordination; reduced line crossings
Bretaud et al., 2004	MPTP	Yes		Yes – Reduced swimming activity	In adults – Respiratory dysfunction and darkened pigmentation.
Bretaud et al., 2004	Paraquat	No		No
Bretaud et al., 2004	Rotenone	No		No
Byrnes et al., 2018	Rotenone	Not reported – But induced brain death phenotype	Reduced skeletal muscle mitochondrial membrane potential	Yes – Reduced responsiveness	Developmental deformities.
Cronin and Grealy, 2017	6-OHDA	Yes		Yes – Reduced locomotor activity	Morphological deformities.
Feng et al., 2014	6-OHDA	Not reported		Yes – Reduced swim distance; increased time spent in the bottom zone of tank
Khotimah et al., 2015	Rotenone	Yes	Synuclein aggregation. Increased apoptosis and caspase expression. Reduced BDNF expression.	Yes – Reduced swim motility
Lam et al., 2005	MPTP	Yes		Yes – Impaired TEER; reduced swim distance and velocity
Martel et al., 2015	Rotenone	Yes	Increased susceptibility to oxidative stress	Yes – Reduced tank midline crossing
McKinley et al., 2005	MPTP	Yes		Not reported
Melo et al., 2015	Rotenone	Not reported		Yes – Impaired TEER; impaired balance; mydriasis; tremors; paralysis; erratic swimming	Developmental deficits. Morphological deformities.
Nellore and Nandita, 2015	Paraquat	reduced dopamine levels; increased cell apoptosis		Yes – Reduced swim distance; impaired tail coiling	Developmental deformities. Increased mortality.
Nunes et al., 2017	Paraquat	Not reported	Reduced mitochondrial viability	Yes – Increased time spent in the top zone of the tank;	Increased aggressiveness.
Sallinen et al., 2009	MPTP	Yes		Yes – Reduced swim distance and speed
Vijayanathan et al., 2017	6-OHDA	Yes		Yes – Reduced swim distance and speed
Wang et al., 2016	Paraquat	Not reported – But increased cell apoptosis	Increased susceptibility to oxidative stress. Increased migration and activation of macrophages.	Not reported	Increased mortality.
Wang et al., 2017	Rotenone	Yes		Yes – Reduced swim duration and distance at fast speed; increased time spent in the light vs. dark	Olfactory dysfunction.
Wang et al., 2018	Paraquat	Not reported	Reduced maximum respiration rate. Increased susceptibility to oxidative stress.	Yes – increased swim distance, duration, and velocity	Accelerated hatching.
Wen et al., 2008	MPTP	Yes		Not reported
Zhang et al., 2015	6-OHDA	Yes		Yes – Reduced swim distance
Zhang et al., 2016	6-OHDA	Yes		Yes – Reduced swim distance