Figure 7.

Intratumoral behavior of DiD-labeled RGDf[N-met]C-liposomes in NMRI nu/nu mice bearing BLM tumor (a–c), and Rho-PE-labeled (red) RGDf[N-met]C-liposomes in C57 mice bearing B16F0 tumor (d–g) imaged by intravital microscopy, using dorsal skin-fold chamber. Panel (h) illustrates the intratumoral behavior of DiD-labeled (purple) plain liposomes. RGDf[N-met]C-liposomes were found to be associated with the tumor vasculature (arrows in a–f) or extravasated and associated with tumor cells and likely other cells in the tumor region (g). Meanwhile, few plain liposomes were found associated with cells inside or outside the tumor vasculatures. Reprinted with permission from [29].