TABLE 1.
Agreement between consensus ELISA and Western blot results for single and sequential specimens
| Specimen type | No. (%) of positive patientsa | No. of blot-positive samplesb | No. (%)c of ELISA samples
|
No. of blot-negative samplesb | No. (%)d of ELISA samples
|
||
|---|---|---|---|---|---|---|---|
| True positive | False negative | True negative | False positive | ||||
| Single (n = 26) | |||||||
| 17-kDa antigen | 24 (92) | 24 | 23e (96) | 1e | 2 | 2e (100) | 0e |
| 27-kDa antigen | 24 (92) | 24 | 24f (100) | 0f | 2 | 2f (100) | 0f |
| Sequential (n = 207; 41 patients) | |||||||
| 17-kDa antigen | 38 (93) | 151 | 111e (74) | 40e | 56 | 49e (87) | 7e |
| 27-kDa antigen | 40 (98) | 186 | 172f (92) | 14f | 21 | 21f (100) | 0f |
a
Number of patients positive for the given antigen by consensus Western blot result, with the percentage of positive patients in parentheses.
b
Consensus of large- and minigel-format Western blot responses.
c
Percentage of Western blot-positive samples correctly identified by consensus ELISA result.
d
Percentage of Western blot-negative samples correctly identified by consensus ELISA result.
e
Triton antigen ELISA detection of 17-kDa antigen.
f
Cp23 ELISA detection of 27-kDa antigen.