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. 2022 Oct 20;10(10):1761. doi: 10.3390/vaccines10101761

Figure 2.

Figure 2

Representative flow cytometry analysis. Cells were gated in an FSC-A vs. Ghost Violet to exclude dead cells; later, in an FSC-A vs. FSC-H dot plot, we excluded doublets. Lymphocyte region was selected using an FSC vs. SSC dot plot. SSC-A vs. CD19 dot plot or SSC-A vs. CD3 followed by CD3 vs. CD4 was made from lymphocyte region. From gated CD19 region, CD27 vs. CD21 plot was made to identify memory (CD21+CD27+) and effector-memory (CD21−CD27+) B-lymphocytes. In addition, expression of FcRL4 was evaluated with histogram from memory and effector-memory B-lymphocytes. On the other hand, from gated CD3, CD4 region, a CXCR5 vs. CCR7 and CD45RO vs. CD45RA plot was made to identify TFH (CCR7−CXCR5+) and memory (CD45RA−CD45RO+) T-lymphocytes, respectively. Analysis of samples from nonvaccinated, vaccinated with the first dose, and vaccinated with the second vaccine dose is shown.