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. 2022 Oct 20;10(10):1761. doi: 10.3390/vaccines10101761

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Longitudinal analysis of B-lymphocytes throughout follow-up vaccination schedule. As of paired samples throughout vaccination scheme (pre-vaccine, first vaccine dose, and second vaccine dose) in COVID-19(−) and recovered COVID-19(+) participants, we analyzed (A) percentages of B-lymphocytes, obtained from SSC-A vs. CD19 plot, and (B) percentages of memory B-lymphocytes and percentages of effector-memory B-lymphocytes, obtained from CD21 vs. CD27 plot gated on CD19+. Moreover, (C) percentages of FcRL4 from memory B-lymphocyte, and percentages of FcRL4 from effector-memory B-lymphocytes, obtained from FcRL4 histogram gated on CD21+CD27+ and CD21−CD27+, respectively, was carried out. Central trend values and dispersion values are indicated in Table S10, Supplementary Data.