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. 2022 Oct 13;14(10):2247. doi: 10.3390/v14102247

Figure 1.

Figure 1

Virological characterization of KHF4 and KHF5. (A): GPs of KHF4 and KHF5 were compared for monoclonal antibody binding. The culture supernatants of hybridoma of monoclonal antibodies were diluted 1:1, 1:10, and 1:100, and positivity in the 1:100, 1:10, and 1:1 dilutions was assessed as +++, ++, and +, respectively. (B) Cell fusion assay. Cell fusion activity of recombinant glycoproteins of KHF4 and KHF5 expressed in Vero E6 cells by transfection (upper panels). Fusion activity of Vero E6 cells infected with KHF4 and KHF5 (central panels). Cells were inoculated with viruses at a MOI of 0.1 and incubated for 5 days. Uninfected Vero E6 cells were used as negative controls (NC; lower panel). (C) Viral growth in Vero E6 cells. Culture supernatant was collected from cells between 1 to 10 dpi, and the viral load was quantified by real-time RT-PCR (Significance was assigned as * p < 0.05, ** p < 0.01, and *** p < 0.001). (D) Pseudotype VSV with KHF4 and KHF5 glycoproteins were transfected to HEK293T cells and the propagated pseudotype VSV and titers were determined in Vero E6 cells.