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Cell tropism of KHF4 and KHF5 in vitro. Viral N protein production was compared by Western blotting to clarify virus replication in various cells. Four cell lines, (Vero E6, A549, U937, and HEK 293T) and peritoneal macrophages, dendritic cells (DC), and spleen cells obtained from five-week-old BALB/c mice were inoculated at a MOI of 0.1 and cultured for 5 days. GAPDH was used as the reference protein.
