Figure 6.

Immunohistochemistry analysis of tissues from KHF4 and KHF5-infected mice. Tissue slices were stained with hematoxylin and eosin (HE), and viral antigens were detected using a biotinylated E5G6 monoclonal antibody with the Vector M.O.M immunodetection kit. Characteristic lesions were indicated by open triangles. Viral antigens are indicated by closed triangles. (A) Peri bronchus edema with lymphocyte aggregation was observed in KHF5-infected lungs, and mild antigen reaction was observed in KHF5-infected alveolar epithelial cells. (B) Petechial hemorrhage was observed in KHF5-infected livers by HE staining, whereas KHF5-infected livers showed multiple antigenic reactions and hemorrhage. The KHF4-infected liver also showed perivascular lymphoid infiltration. (C) Renal hemorrhage caused by KHF5 infection was distributed in the cortex-medullary region, and antigens were detected in the tubular regions.