Table 4.
Cytotoxicity of compounds 47–50, 53, 54, 57, 59, 60–63, 65–70, and 72 on cell cycle in CCRF-CEM lymphoblasts.
| % of Total Cellular Populations | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| No | Conc. (µM) |
Sub-G1 Apoptosis |
G0/G1 | S | G2/M | >G2/M | M a | DNA b Synthesis |
RNA b Synthesis |
| 47 | 2.74 13.7 |
3.36 7.59 |
37.93 27.73 |
43.41 39.33 |
18.66 32.94 |
9.71 10.56 |
1.87 8.29 |
2.76 24.01 |
19.4 24.95 |
| 48 | 1.42 7.1 |
2.83 4.37 |
34.46 49.43 |
46.76 23.5 |
18.78 27.08 |
9.95 12.21 |
2.11 2.47 |
8.1 6.21 |
16.56 16.73 |
| 49 | 1.34 6.7 |
3.14 5.14 |
35.52 40.24 |
43.74 36.15 |
20.74 23.61 |
13.16 10.02 |
2.09 3.35 |
18.2 5.93 |
19.14 17.12 |
| 50 | 1.32 6.6 |
5.09 5.83 |
35.7 49.76 |
47.92 30.92 |
16.38 19.32 |
14.2 9.11 |
1.42 2.12 |
39.66 15.47 |
26.23 20.85 |
| 53 | 1.31 6.55 |
4.52 6.93 |
33.63 39.14 |
47.13 42.84 |
19.24 18.01 |
10.22 7.45 |
1.74 1.57 |
39.73 26.21 |
57 27.33 |
| 54 | 1.09 5.45 |
5.68 7.28 |
35.54 50.08 |
43.61 31.69 |
20.85 18.23 |
9.52 7.91 |
2.02 3.21 |
29.33 20.4 |
28.71 17.96 |
| 57 | 1.51 7.55 |
4.62 5.7 |
36 43 |
37.02 30.14 |
26.98 26.85 |
13.61 12.5 |
1.78 2.12 |
18.86 19.87 |
10.43 9.22 |
| 59 | 1.2 6 |
8.8 9.63 |
34.06 42.51 |
47.12 33.63 |
18.82 23.85 |
10.37 10.89 |
1.52 5.65 |
41.37 10.43 |
47.78 34.65 |
| 60 | 1.13 5.65 |
4.28 6.97 |
34.48 41.01 |
46.3 31.97 |
19.23 27.02 |
11.73 11.18 |
2.63 4.67 |
32.46 15.1 |
26.93 11.19 |
| 61 | 1.05 5.25 |
3.69 10.05 |
33.19 32.98 |
47.6 44.88 |
19.21 22.14 |
11.24 12.98 |
2.03 2.24 |
34.82 9.05 |
29.57 7.41 |
| 62 | 1.17 5.85 |
13.92 8.12 |
27.68 37.23 |
44.18 43.17 |
28.15 19.61 |
9.65 8.22 |
1.38 1.5 |
32.88 17.61 |
10.51 30.99 |
| 63 | 0.77 3.85 |
3.41 12.97 |
42.76 34.13 |
40.42 47.99 |
16.81 17.88 |
8.56 11.38 |
1.66 1.53 |
41.06 13.54 |
35.01 2.79 |
| 65 | 0.55 2.75 |
17.77 8.67 |
40.82 47.21 |
39.14 32.8 |
20.04 19.99 |
7.55 7.13 |
1 1.66 |
36.82 19.73 |
36.04 24.95 |
| 66 | 1.11 5.55 |
2.95 11.72 |
39.53 48.53 |
4387 24.9 |
16.6 26.56 |
10.42 10.45 |
1.64 2.29 |
39.87 9.68 |
29.44 3.05 |
| 67 | 1.05 5.25 |
3.13 11.06 |
37.85 40.09 |
43.93 40.3 |
18.22 19.61 |
9.56 8.98 |
1.59 2.27 |
42.05 9.76 |
38.15 6.99 |
| 68 | 1.23 6.15 |
2.71 7.16 |
35.73 48.5 |
45.73 31.86 |
18.54 19.64 |
9.87 9.65 |
1.73 2.11 |
41.27 7.18 |
37.4 3.8 |
| 69 | 1.38 6.9 |
3.3 13.89 |
43.48 38.16 |
35.7 42.58 |
20.83 19.27 |
8.58 12.52 |
1.61 1.6 |
34.45 4.22 |
31.54 2.64 |
| 70 | 1.16 5.8 |
3.21 13.75 |
39.54 33.44 |
38.46 43.31 |
21.99 23.25 |
10.54 10.33 |
1.57 1.08 |
35.58 4.88 |
35.28 2.88 |
| 72 | 2.14 10.7 |
4.05 10.12 |
47.22 31.36 |
34.32 46.65 |
18.46 21.98 |
8.35 13.12 |
1.61 5.28 |
27.07 4.35 |
21.64 2.19 |
| Control | 0 | 2.91 | 36.69 | 44.89 | 18.42 | 9.38 | 1.68 | 36.52 | 32.97 |
a Mitosis Phospho-Histone3 (Ser10). b DNA and RNA synthesis in CCRF-CEM lymphoblasts (% positive of cells). Flow cytometry analysis was used to quantify cell cycle distribution and percentage of apoptotic cells. The sum of the percentage for G0/G1, S, and G2/M is equal to 100%.