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. 2022 Sep 30;14(10):2157. doi: 10.3390/v14102157

Figure 4.

Figure 4

Selectivity of Ad-LVs in co-culture assay using different transduction protocols. To confirm selectivity of Ad-LVs, target cells were transduced with 0.2 TU/cell in a co-culture with non-target cells. Cell-trace-dye-labeled SupT1 cells (CD4+, CD8+, and CD46+) were co-cultivated in a 1:1 ratio with Raji cells (CD19, CD20+). (A) Representative data are shown using spinoculation. The co-culture was left untreated (-) or was transduced with Ad-LVs in the presence of non-biotinylated mAB (-) or LLE-CD4 mAB (clone: MT-466, 50 ng/mL), LLE-CD8 mAB (clone: BW135/80, 10 ng/mL) or LLE-CD20 mAB (clone: LT20, 500 ng/mL). (B) Three transduction conditions were compared using no enhancer, using the transduction enhancer Vectofusin®-1, or using spinoculation. Transduction efficiency was evaluated 4 days post transduction via gating on the cell-trace-dye-labeled SupT1 and the CD19-expressing Raji cells. Data are represented as mean ± SD of 3 technical replicates.