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. 2022 Sep 30;14(10):2157. doi: 10.3390/v14102157

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Selective transduction of activated murine T cells. Murine T cells were isolated from murine spleens, activated overnight, and transduced using Ad-LVs at a dose of 5 TU/cell. (A) Representative data are shown of CD4+ T cells transduced in the absence of adapter or in the presence of LLE-CD4 fab. (B) Isolated murine T cells from spleen were either left untreated (-) or transduced with Ad-LVs in the absence of adapter (-) or in the presence of LLE-CD4 fab (clone: GK1.5) and LLE-CD8 fab (clone: 53–6.7)-specific adapter molecules (500 ng/mL). VSV-G LVs were used as positive controls. Data are represented as mean ± SD of 3 different mice.