Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Representation of the HDR template design for a (A) few base-pair point mutations, (B) large deletion, (C) short (≤80 bp) insertion, or (D) long (>80 bp) insertion into the yeast genome. Note: as indicated in Section 3.1, a silent mutation of the PAM (or another other portion of the gRNA target sequence) should be always included if the mutation does not otherwise disrupt the gRNA target, to prevent repeated cutting by Cas9 after repair is complete.
