Table 4.

Intestinal epithelial cell endogenous IL-10 activity regulates bacterial sensing molecules dependent on inflammatory stimulus.

Readout/Treatment	Control	+anti-IL-10	p Values
TLR4 mRNA—Unstim	1.0 ± 0	50 ± 1	<0.001 ***
+TNFα	5 ± 0.4	20 ± 0.5	<0.05 *
+IL-1β	20 ± 1	100 ± 1	<0.05 *
TLR2 mRNA—Unstim	1.0 ± 0	100 ± 5	<0.001 ***
+TNFα	5 ± 1	500 ± 10	<0.001 ***
+IL-1β	5 ± 1	900 ± 15	<0.001 ***
CD14 mRNA—Unstim	1.0 ± 0	70 ± 2	<0.001 ***
+TNFα	5 ± 1	120 ± 3	<0.001 ***
+IL-1β	70 ± 2	70 ± 1	ns
MD2 mRNA—Unstim	1.0 ± 0	3 ± 1	<0.05 *
+TNFα	0.8 ± 0.2	3 ± 1	<0.05 *
+IL-1β	4 ± 1.5	2 ± 1	<0.05 *
NOD2 mRNA—Unstim	1.0 ± 0	50 ± 6	<0.001 ***
+TNFα	15 ± 1	150 ± 5	<0.01 **
+IL-1β	70 ± 2	500 ± 10	<0.001 ***
TLR9 mRNA—Unstim	1.0 ± 0	90 ± 2	<0.001 ***
+TNFα	30 ± 2	110 ± 10	<0.001 ***
+IL-1β	3 ± 1	30 ± 4	<0.001 ***
Tollip mRNA—Unstim	1.0 ± 0	0.001 ± 0.0002	<0.001 ***
+TNFα	0.05 ± 0.002	0.10 ± 0.04	<0.05 *
+IL-1β	0.01 ± 0.001	0.10 ± 0.03	<0.05 *

Caco-2 epithelial cells were pre-treated with or without 10 μgmL⁻¹ anti-IL-10 neutralising antibody for 4 h, followed by stimulation with or without 10 ngmL⁻¹ IL-1β or 10 ngmL⁻¹ TNFα for a further 18 h. Induction of bacterial sensing molecule mRNA expression (TLR4, TLR2, CD14, MD2, NOD2, TLR9 & Tollip) is presented as relative expression (RQ, Arbitary Units) compared to GAPDH housekeeping gene expression. Data displayed is a representative experiment with triplicate samples for n = 3 replicate experiments. Significant effects of endogenous IL-10 are compared to the respective unstimulated or cytokine-stimulated control and significance indicated as * p < 0.05, ** p < 0.01, *** p < 0.001 and ns, not significant.