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Gradient UHPLC quantification method for cysteamine and cystamine. The mobile phase A was an aqueous solution of 4 mM sodium heptane sulfonate, and the mobile phase B was acetonitrile.
| Time | Mobile Phase A (%) | Mobile Phase B (%) |
|---|---|---|
| 0 | 95.0 | 5.0 |
| 0.50 | 95.0 | 5.0 |
| 2.50 | 70.0 | 30.0 |
| 3.20 | 95.0 | 5.0 |
| 6.0 | 95.0 | 5.0 |
